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Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene.cite-ref-pmid9651582-5-0[5]cite-ref-pmid9224719-6-0[6]cite-ref-entrez-7-0[7]

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs.

In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs.

Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.cite-ref-entrez-7-1[7]

• References

• Further reading

• External links

cite-note-refgrch38ensembl-11. GRCh38: Ensembl release 89: ENSG00000160917 – Ensembl, May 2017

cite-note-refgrcm38ensembl-22. GRCm38: Ensembl release 89: ENSMUSG00000029625 – Ensembl, May 2017

cite-note-33. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

cite-note-44. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

cite-note-pmid9651582-55. ↑ citerefnemeroffbarabinolikeller1998Nemeroff ME, Barabino SM, Li Y, Keller W, Krug RM (Jul 1998). "Influenza virus NS1 protein interacts with the cellular 30 kDa subunit of CPSF and inhibits 3'end formation of cellular pre-mRNAs". Mol Cell. 1 (7): 991–1000. doi:10.1016/S1097-2765(00)80099-4. PMID 9651582.

cite-note-pmid9224719-66. ↑ citerefbarabinohubnerjennyminvielle-sebastia1997Barabino SM, Hubner W, Jenny A, Minvielle-Sebastia L, Keller W (Aug 1997). "The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins". Genes Dev. 11 (13): 1703–16. doi:10.1101/gad.11.13.1703. PMID 9224719.

cite-note-entrez-77. ↑ "Entrez Gene: CPSF4 cleavage and polyadenylation specific factor 4, 30kDa".

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• Human CPSF4 genome location and CPSF4 gene details page in the UCSC Genome Browser.

• Overview of all the structural information available in the PDB for UniProt: O95639 (Cleavage and polyadenylation specificity factor subunit 4) at the PDBe-KB.